Nup153 monoclonal antibody, clone R4C8
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More Files
- More Functions
Western Blot (Cell lysate)
Detection of Nup153 by Western blotting with Nup153 monoclonal antibody, clone R4C8 (Cat # MAB6759). Sample is the nuclear membrane fraction of HeLa cells (Lane 1) and NIH/3T3 cells (Lane 2).
Immunofluorescence
Immunofluorescent staining of HeLa cells with Nup153 monoclonal antibody, clone R4C8 (Cat # MAB6759).
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Specification
Product Description
Rat monoclonal antibody raised against partial recombinant Nup153.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 610-1191 rat Nup153.
Host
Rat
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG2b, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Detection of Nup153 by Western blotting with Nup153 monoclonal antibody, clone R4C8 (Cat # MAB6759). Sample is the nuclear membrane fraction of HeLa cells (Lane 1) and NIH/3T3 cells (Lane 2).Immunocytochemistry
Immunofluorescence
Immunofluorescent staining of HeLa cells with Nup153 monoclonal antibody, clone R4C8 (Cat # MAB6759).Enzyme-linked Immunoabsorbent Assay
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Gene Info — Nup153
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Publication Reference
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Nanomechanical basis of selective gating by the nuclear pore complex.
Lim RY, Fahrenkrog B, Koser J, Schwarz-Herion K, Deng J, Aebi U.
Science 2007 Oct; 318(5850):640.
Application：IEM, Human, Nuclear pore complexes.
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The nucleoporin nup153 plays a critical role in multiple types of nuclear export.
Ullman KS, Shah S, Powers MA, Forbes DJ.
Molecular Biology of the Cell 1999 Mar; 10(3):649.
Application：Func, Frog, Oocytes.
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Nanomechanical basis of selective gating by the nuclear pore complex.
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