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Last updated: 2017/10/15
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HBEGF monoclonal antibody, clone 4G10

  • Catalog # : MAB6756
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against recombinant HBEGF.
  • Immunogen:
  • Recombinant protein corresponding to human HBEGF.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • Western Blot (0.2-1 ug/mL)
    Immunofluorescence (5~10 ug/mL)
    Immunoprecipitation (2 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (50% glycerol, azide free)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blotting with HBEGF monoclonal antibody, clone 4G10 (Cat # MAB6756).
    Lane 1. Vero cell extract.
    2. Vero cells carrrying human HBEGF expression vector.
    3. Vero cells carrying mouse HB-EGF expression vector.
  • Immunocytochemistry
  • Immunocytochemistry
  • Immunocytochemical staining with HBEGF monoclonal antibody, clone 4G10 (Cat # MAB6756).
    A. VERO cells carrying human HGEGF expression vector.
    B. Vero cells carrying mouse HB-EGF expression vector.
    Cells treated with antibody 4G10, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation with HBEGF monoclonal antibody, clone 4G10 (Cat # MAB6756).
    Lane 1. Vero cell extract.
    2. Vero cells carrrying human HBEGF expression vector.
    3. Vero cells carrying mouse HB-EGF expression vector.
    Except that the cell surface was biotinylated.
    After immunoprecipitation with the antibody, HRP-conjugated streptoavidin was used to detect the HBEGF by Western blotting.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1839
  • Gene Name:
  • HBEGF
  • Gene Alias:
  • DTR,DTS,DTSF,HEGFL
  • Gene Description:
  • heparin-binding EGF-like growth factor
  • Other Designations:
  • diphtheria toxin receptor (heparin-binding EGF-like growth factor),diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor),heparin-binding epidermal growth factor
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