PHLDA3 monoclonal antibody, clone 4B6
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More Files
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Western Blot (Cell lysate)
Western blotting was performed using PHLDA3 monoclonal antibody, clone 4B6 (Cat # MAB6755) to detect PHLDA3 protein expression. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right).
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Specification
Product Description
Rat monoclonal antibody raised against synthetic peptide of PHLDA3.
Immunogen
A synthetic peptide corresponding to amino acids 1-31 of human PHLDA3.
Host
Rat
Reactivity
Human
Specificity
This antibody is specific to human PHLDA3.
Form
Liquid
Isotype
IgG2b, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (50% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blotting was performed using PHLDA3 monoclonal antibody, clone 4B6 (Cat # MAB6755) to detect PHLDA3 protein expression. PHLDA3 protein was induced by Ad-p53 in MDA-MB-468 cells (left). PHLDA3 expression was inhibited by shRNA targeting PHLDA3 (right). -
Gene Info — PHLDA3
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Interactome
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Publication Reference
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PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.
Kawase T, Ohki R, Shibata T, Tsutsumi S, Kamimura N, Inazawa J, Ohta T, Ichikawa H, Aburatani H, Tashiro F, Taya Y.
Cell 2009 Feb; 136(3):535.
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A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting.
Frank D, Mendelsohn CL, Ciccone E, Svensson K, Ohlsson R, Tycko B.
Mammalian Genome 1999 Dec; 10(12):1150.
Application：WB-Ti, Mouse, Adult mouse tissues.
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PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.
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