EID1 monoclonal antibody, clone #26
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More Files
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Western Blot (Transfected lysate)
Identification of the EID1 protein by EID1 monoclonal antibody, clone #26 (Cat # MAB6753) by western blotteing.
Crude cell extracts of MCF-7 cells (breast cancer cell line) transfected with control vector pCMV1 (Lane 1) or the EID1 expression vector pcDNA3/EID1 (Lane 2).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of EID1.
Immunogen
A synthetic peptide corresponding to amino acids 159-187 of human EID1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
This antibody reacts with EID1.
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Transfected lysate)
Identification of the EID1 protein by EID1 monoclonal antibody, clone #26 (Cat # MAB6753) by western blotteing.
Crude cell extracts of MCF-7 cells (breast cancer cell line) transfected with control vector pCMV1 (Lane 1) or the EID1 expression vector pcDNA3/EID1 (Lane 2).Enzyme-linked Immunoabsorbent Assay
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Gene Info — EID1
Entrez GeneID
23741Gene Name
EID1
Gene Alias
C15orf3, CRI1, EID-1, IRO45620, MGC138883, MGC138884, PNAS-22, PTD014, RBP21
Gene Description
EP300 interacting inhibitor of differentiation 1
Omim ID
605894Gene Ontology
HyperlinkOther Designations
CREBBP/EP300 inhibitor 1|CREBBP/EP300 inhibitory protein 1|NB4 apoptosis related protein|Rb- and p300-binding protein EID-1|retinoblastoma protein-associated protein
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Interactome
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Publication Reference
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Acetylation regulates the differentiation-specific functions of the retinoblastoma protein.
Nguyen DX, Baglia LA, Huang SM, Baker CM, McCance DJ.
The EMBO Journal 2004 Apr; 23(7):1609.
Application：WB, Mouse, C2C12 cells.
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A novel Rb- and p300-binding protein inhibits transactivation by MyoD.
MacLellan WR, Xiao G, Abdellatif M, Schneider MD.
Molecular and Cellular Biology 2000 Dec; 20(23):8903.
Application：IP, WB-Tr, Human, Skeletal myoblasts, U2OS cells.
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Acetylation regulates the differentiation-specific functions of the retinoblastoma protein.
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