KDM5A monoclonal antibody, clone 9A6
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More Files
- More Functions
Western Blot (Cell lysate)
Identification of KDM5A in crude cell extracts by western blotting with KDM5A monoclonal antibody, clone 9A6 (Cat # MAB6751).
1. HeLa control siRNA
2. HeLa KDM5A siRNA
3. MCF-7
4. U-2 OS
5. NIH/3T3
6. J1 (mouse ES)
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of KDM5A.
Immunogen
A synthetic peptide corresponding to amino acids 1416-1434 of human KDM5A.
Host
Mouse
Reactivity
Human
Specificity
This antibody is specific to human and mouse KDM5A. It can detect endogenous levels of KDM5A.
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Identification of KDM5A in crude cell extracts by western blotting with KDM5A monoclonal antibody, clone 9A6 (Cat # MAB6751).
1. HeLa control siRNA
2. HeLa KDM5A siRNA
3. MCF-7
4. U-2 OS
5. NIH/3T3
6. J1 (mouse ES) -
Gene Info — JARID1A
Entrez GeneID
5927Gene Name
JARID1A
Gene Alias
KDM5A, RBBP2, RBP2
Gene Description
jumonji, AT rich interactive domain 1A
Omim ID
180202Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
Jumonji, AT rich interactive domain 1A (RBBP2-like)|Jumonji, AT rich interactive domain 1A (RBP2-like)|retinoblastoma binding protein 2|retinoblastoma-binding protein 2
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Interactome
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Publication Reference
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Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation.
Lopez-Bigas N, Kisiel TA, Dewaal DC, Holmes KB, Volkert TL, Gupta S, Love J, Murray HL, Young RA, Benevolenskaya EV.
Molecular Cell 2008 Aug; 31(4):520.
Application：ChIP, Human, U-937 cells.
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RBP2 belongs to a family of demethylases, specific for tri-and dimethylated lysine 4 on histone 3.
Christensen J, Agger K, Cloos PA, Pasini D, Rose S, Sennels L, Rappsilber J, Hansen KH, Salcini AE, Helin K.
Cell 2007 Mar; 128(6):1063.
Application：IP, WB-Re, Recombinant protein.
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The retinoblastoma binding protein RBP2 is an H3K4 demethylase.
Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG Jr.
Cell 2007 Mar; 128(5):889.
Application：WB-Ce, Mouse, MEFs.
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Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation.
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