PRR7 monoclonal antibody, clone TRAP3/10
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More Files
- More Functions
Western Blot (Tissue lysate)
Detection of PRR7 in murine brain lysate by Western blotting using PRR7 monoclonal antibody, clone TRAP3/10 (Cat # MAB6689).
Immunofluorescence
Immunofluorescence staining of HEK-293 cells cotransfected with PRR7 (red) and GFP-PSD-95 (green). PRR7 detected by PRR7 monoclonal antibody, clone TRAP3/10 (Cat # MAB6689). DNA visualized by DAPI (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant PRR7.
Immunogen
Recombinant protein corresponding to amino acids 126-253 of human PRR7.
Host
Mouse
Theoretical MW (kDa)
28
Reactivity
Human, Mouse
Specificity
This antibody recognizes PRR7, a 28 KDa proline-rich membrane protein presumably associated with NMDA receptor complex.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG2a
Recommend Usage
Immunocytochemistry (10 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Detection of PRR7 in murine brain lysate by Western blotting using PRR7 monoclonal antibody, clone TRAP3/10 (Cat # MAB6689).Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of HEK-293 cells cotransfected with PRR7 (red) and GFP-PSD-95 (green). PRR7 detected by PRR7 monoclonal antibody, clone TRAP3/10 (Cat # MAB6689). DNA visualized by DAPI (blue). -
Gene Info — PRR7
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Interactome
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Publication Reference
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Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS.
Nature Communications 2018 Aug; 9(1):3434.
Application：IF, WB-Tr, Human, Mouse, Hippocampal neuron, HEK293 cells.
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Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
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