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Last updated: 2016/11/27
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L-Cysteine monoclonal antibody, clone F2D

  • Catalog # : MAB6677
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against L-Cysteine.
  • Immunogen:
  • Cysteine conjugated with KLH.
  • Host:
  • Mouse
  • Form:
  • Liquid
  • Isotype:
  • IgG2a
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.2 (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Porcine peripheral red blood cells were paraffn embedded and sectioned. 4 um sections were de-paraffnized and rehydrated. Sections were incubated with or without additional thiol compounds and L-Cysteine monoclonal antibody, clone F2D (Cat # MAB6677) for 2 hours at room temperature. Antibody concentration was 3 ug/mL . Sections were blocked pre- and post antibody treatements with 1% BSA in PBS. Development was with a goat anti-mouse IgG antibody labeled with Alexa™ 488.
  • Immunofluorescence
  • Immunofluorescence
  • Cysteine addition was carried out at 20 mM in 1% BSA in PBS and added with L-Cysteine monoclonal antibody, clone F2D (Cat # MAB6677) at 3 ug/mL for 1 hour at room temperature. With polymorphonuclear neutrophils addition of 20 mM cytseine reduced or eliminated the goat anti-mouse IgG-Alexa 488 signal Nuclear staining was carried out with TOPRO at 1 : 500 dilution in PBS.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
  • ELISA
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