L-Cysteine monoclonal antibody, clone F2D
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More Files
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Immunofluorescence
Porcine peripheral red blood cells were paraffn embedded and sectioned. 4 um sections were de-paraffnized and rehydrated. Sections were incubated with or without additional thiol compounds and L-Cysteine monoclonal antibody, clone F2D (Cat # MAB6677) for 2 hours at room temperature. Antibody concentration was 3 ug/mL . Sections were blocked pre- and post antibody treatements with 1% BSA in PBS. Development was with a goat anti-mouse IgG antibody labeled with Alexa™ 488.
Immunofluorescence
Cysteine addition was carried out at 20 mM in 1% BSA in PBS and added with L-Cysteine monoclonal antibody, clone F2D (Cat # MAB6677) at 3 ug/mL for 1 hour at room temperature. With polymorphonuclear neutrophils addition of 20 mM cytseine reduced or eliminated the goat anti-mouse IgG-Alexa 488 signal Nuclear staining was carried out with TOPRO at 1 : 500 dilution in PBS.
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Specification
Product Description
Mouse monoclonal antibody raised against L-Cysteine.
Immunogen
Cysteine conjugated with KLH.
Host
Mouse
Form
Liquid
Isotype
IgG2a
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Porcine peripheral red blood cells were paraffn embedded and sectioned. 4 um sections were de-paraffnized and rehydrated. Sections were incubated with or without additional thiol compounds and L-Cysteine monoclonal antibody, clone F2D (Cat # MAB6677) for 2 hours at room temperature. Antibody concentration was 3 ug/mL . Sections were blocked pre- and post antibody treatements with 1% BSA in PBS. Development was with a goat anti-mouse IgG antibody labeled with Alexa™ 488.Immunofluorescence
Cysteine addition was carried out at 20 mM in 1% BSA in PBS and added with L-Cysteine monoclonal antibody, clone F2D (Cat # MAB6677) at 3 ug/mL for 1 hour at room temperature. With polymorphonuclear neutrophils addition of 20 mM cytseine reduced or eliminated the goat anti-mouse IgG-Alexa 488 signal Nuclear staining was carried out with TOPRO at 1 : 500 dilution in PBS.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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