Hcn1 monoclonal antibody, clone S70
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More Files
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Immunofluorescence
Immunofluorescence staining in human hippocampus with Hcn1 monoclonal antibody, clone S70 (Cat # MAB6651).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant Hcn1.
Immunogen
Recombinant fusion protein corresponding to C-terminus amino acids 778-910 of rat Hcn1.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
Detects ~100KDa. No cross-reactivity against HCN2.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1-10 ug/mL)
Immunohistochemistry (0.1-1.0 ug/mL)
Immunocytochemistry (0.1-1.0 ug/mL)
Immunofluorescence (1.0-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescence staining in human hippocampus with Hcn1 monoclonal antibody, clone S70 (Cat # MAB6651).Immunoprecipitation
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Gene Info — Hcn1
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Publication Reference
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Discrete subicular circuits control generalization of hippocampal seizures.
Fan Fei, Xia Wang, Cenglin Xu, Jiaying Shi, Yiwei Gong, Heming Cheng, Nanxi Lai, Yeping Ruan, Yao Ding, Shuang Wang, Zhong Chen, Yi Wang.
Nature Communications 2022 Aug; 13(1):5010.
Application：IF, Mouse, Mouse brain.
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Deep posteromedial cortical rhythm in dissociation.
Sam Vesuna, Isaac V Kauvar, Ethan Richman, Felicity Gore, Tomiko Oskotsky, Clara Sava-Segal, Liqun Luo, Robert C Malenka, Jaimie M Henderson, Paul Nuyujukian, Josef Parvizi, Karl Deisseroth.
Nature 2020 Oct; 586(7827):87.
Application：IF, IHC, Mouse, Mouse brain.
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Congenital long QT syndrome.
Crotti L, Celano G, Dagradi F, Schwartz PJ.
Orphanet Journal of Rare Diseases 2008 Jul; 3:18.
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Ca(V)1.2 calcium channel dysfunction causes a multisystem disorder including arrhythmia and autism.
Splawski I, Timothy KW, Sharpe LM, Decher N, Kumar P, Bloise R, Napolitano C, Schwartz PJ, Joseph RM, Condouris K, Tager-Flusberg H, Priori SG, Sanguinetti MC, Keating MT.
Cell 2004 Oct; 119(1):19.
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Discrete subicular circuits control generalization of hippocampal seizures.
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