CRYAB monoclonal antibody, clone 3A10.C9
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Specification
Product Description
Mouse monoclonal antibody raised against native CRYAB.
Immunogen
Native purified CRYAB.
Host
Mouse
Reactivity
Bovine, Human
Specificity
Detects a band at ~20KDa (predicted mol.weight is ~21KDa) corresponding to alphaB-crystallin. Does not cross-react with alphaA-crystallin, betaL-crystallin, betaH-crystallin, gamma-crystallin, Hsp25, Hsp27 or Hsp47 proteins.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis from cell and tissue lysates with CRYAB monoclonal antibody, clone 3A10.C9 (Cat # MAB6643).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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alpha-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress.
Yaung J, Jin M, Barron E, Spee C, Wawrousek EF, Kannan R, Hinton DR.
Molecular Vision 2007 Apr; 13:566.
Application:IEM, WB, Human, Human retinal pigment epithelium.
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Association of the chaperone alphaB-crystallin with titin in heart muscle.
Bullard B, Ferguson C, Minajeva A, Leake MC, Gautel M, Labeit D, Ding L, Labeit S, Horwitz J, Leonard KR, Linke WA.
The Journal of Biological Chemistry 2004 Feb; 279(9):7917.
Application:Dot, IF, WB, Rabbit, Rabbit cardiac myofibrils.
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Alpha-crystallin.
Horwitz J.
Experimental Eye Research 2003 Feb; 76(2):145.
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alpha-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress.
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