Ubiquitin monoclonal antibody, clone 5B9-B3
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More Files
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Western Blot (Cell lysate)
Western blot analysis from human cell line muixed lysate with Ubiquitin monoclonal antibody, clone 5B9-B3 (Cat # MAB6641).
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Specification
Product Description
Mouse monoclonal antibody raised against native Ubiquitin.
Immunogen
Native purified bovine Ubiquitin (conjugated with KLH).
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat
Specificity
This antibody recognizes ~10KDa corresponding to free ubiquitin
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis from human cell line muixed lysate with Ubiquitin monoclonal antibody, clone 5B9-B3 (Cat # MAB6641).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Ubiquitin and the control of protein fate in the secretory and endocytic pathways.
Bonifacino JS, Weissman AM.
Annual Review of Cell and Developmental Biology 1998 Nov; 14:19.
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Novel aspects of degradation of T cell receptor subunits from the endoplasmic reticulum (ER) in T cells: importance of oligosaccharide processing, ubiquitination, and proteasome-dependent removal from ER membranes.
Yang M, Omura S, Bonifacino JS, Weissman AM.
The Journal of Experimental Medicine 1998 Mar; 187(6):835.
Application：WB-Ce, Mouse, BW5147 cells.
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Roles of ubiquitinylation in proteolysis and cellular regulation.
K D Wilkinson.
Annual Review of Nutrition 1995 Jan; 15:161.
Application：Blocking, IP, Human, Human lymphocytes, Recombinant proteins.
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Ubiquitin and the control of protein fate in the secretory and endocytic pathways.
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