DNA Damage monoclonal antibody, clone 15A3
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More Files
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Immunofluorescence
Immunofluorescence staining of ischemic rat brain tissue with DNA Damage monoclonal antibody, clone 15A3 (Cat # MAB6638).
Courtesy of Dr. Yi Yang, U. New Mexico.
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Specification
Product Description
Mouse monoclonal antibody raised against DNA Damage.
Immunogen
8-hydroxy-guanosine-BSA and -casein conjugates.
Host
Mouse
Specificity
Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunofluorescence
Immunofluorescence staining of ischemic rat brain tissue with DNA Damage monoclonal antibody, clone 15A3 (Cat # MAB6638).
Courtesy of Dr. Yi Yang, U. New Mexico.Enzyme-linked Immunoabsorbent Assay
Purification
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Publication Reference
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Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.
Chaitali Mukherjee, Tina Kling, Belisa Russo, Kerstin Miebach, Eva Kess, Martina Schiffere, Liliana D Pedro, Ulrich Weikert, Maryam K Fard, Nirmal Kannaiyan, Moritz Rossner, Marie-Louise Aicher, Sandra Goebbels, Klaus-Armin Nave, Eva-Maria Krämer-Albers, Anja Schneider, Mikael Simons.
Cell Metabolism 2020 Aug; 32(2):259.
Application：IF, Mouse, Brain.
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8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures.
Pilger A, Rudiger HW.
International Archives of Occupational and Environmental Health 2006 Oct; 80(1):1.
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Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells.
Kim HW, Murakami A, Williams MV, Ohigashi H.
Carcinogenesis 2003 Feb; 24(2):235.
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Oxidative DNA damage precedes DNA fragmentation after experimental stroke in rat brain.
Cui J, Holmes EH, Greene TG, Liu PK.
FASEB Journal 2000 May; 14(7):955.
Application：IF, IHC-Fr, Rat, Rat brains.
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Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain.
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