HSPA8 monoclonal antibody, clone 1F2-H5
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More Files
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Western Blot
Western blot analysis of HSPA8 monoclonal antibody, clone 1F2-H5 (Cat # MAB6636).
Lane 1, human cell line mix.
Lane 2, recombinant rHsp70 protein.
Lane 3, recombinant rHsc70 protein.
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Specification
Product Description
Mouse monoclonal antibody raised against HSPA8.
Immunogen
Human HSPA8.
Host
Mouse
Reactivity
Human, Mouse, Rat
Specificity
Detects ~73KDa. Does not cross react with Hsp70.
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of HSPA8 monoclonal antibody, clone 1F2-H5 (Cat # MAB6636).
Lane 1, human cell line mix.
Lane 2, recombinant rHsp70 protein.
Lane 3, recombinant rHsc70 protein.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — HSPA8
Entrez GeneID
3312Gene Name
HSPA8
Gene Alias
HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71
Gene Description
heat shock 70kDa protein 8
Omim ID
600816Gene Ontology
HyperlinkGene Summary
The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq
Other Designations
LPS-associated protein 1|N-myristoyltransferase inhibitor protein 71|constitutive heat shock protein 70|heat shock 70kD protein 8|heat shock 70kd protein 10|heat shock cognate protein 54|heat shock cognate protein, 71-kDa|lipopolysaccharide-associated pro
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Interactome
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Pathway
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Disease
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Publication Reference
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β-TrCP upregulates HIF-1 in prostate cancer cells.
Cohen M, Amir S, Golan M, Ben-Neriah Y, Mabjeesh NJ.
Prostate 2019 Mar; 79(4):403.
Application：IP, WB, Human, PC-3, LNCaP cells.
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Molecular chaperones and the centrosome. A role for HSP 73 in centrosomal repair following heat shock treatment.
Brown CR, Hong-Brown LQ, Doxsey SJ, Welch WJ.
The Journal of Biological Chemistry 1996 Jan; 271(2):833.
Application：Func, IF, Monkey, COS cells.
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Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis.
DeLuca-Flaherty C, McKay DB, Parham P, Hill BL.
Cell 1990 Sep; 62(5):875.
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Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells.
Rothman JE.
Cell 1989 Nov; 59(4):591.
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β-TrCP upregulates HIF-1 in prostate cancer cells.
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