Lamp2 monoclonal antibody, clone GL2A7
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More Files
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Immunofluorescence
Immunofluorescence showing distribution of lysosomes in CEC cells with Lamp2 monoclonal antibody, clone GL2A7 (Cat # MAB6635).
Courtesy of Eunduck E.P. Kay, Doheny Eye Institue
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Specification
Product Description
Mouse monoclonal antibody raised against native Lamp2.
Immunogen
Native purified Lamp2 from mouse liver.
Host
Mouse
Reactivity
Mouse, Rabbit
Specificity
This antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunocytochemistry (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescence showing distribution of lysosomes in CEC cells with Lamp2 monoclonal antibody, clone GL2A7 (Cat # MAB6635).
Courtesy of Eunduck E.P. Kay, Doheny Eye InstitueImmunoprecipitation
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Gene Info — Lamp2
Entrez GeneID
16784Gene Name
Lamp2
Gene Alias
CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3
Gene Description
lysosomal-associated membrane protein 2
Gene Ontology
HyperlinkOther Designations
Lamp II|OTTMUSP00000018739|OTTMUSP00000018801|lysosomal membrane glycoprotein 2|lysosome-associated membrane protein-2
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Publication Reference
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Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.
Tanaka Y, Guhde G, Suter A, Eskelinen EL, Hartmann D, Lullmann-Rauch R, Janssen PM, Blanz J, von Figura K, Saftig P.
Nature 2000 Aug; 406(6798):902.
Application：WB-Ti, Mouse, Liver, Kidney.
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A di-leucine-based motif in the cytoplasmic tail of LIMP-II and tyrosinase mediates selective binding of AP-3.
Honing S, Sandoval IV, von Figura K.
The EMBO Journal 1998 Aug; 17(5):1304.
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Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium.
Kannan K, Stewart RM, Bounds W, Carlsson SR, Fukuda M, Betzing KW, Holcombe RF.
Cellular Immunology 1996 Jul; 171(1):10.
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Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice.
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