Lamp1 monoclonal antibody, clone Ly1C6
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More Files
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Immunofluorescence
Immunofluorescence staining in transfected HeLa cells with Lamp1 monoclonal antibody, clone Ly1C6 (Cat # MAB6634).
Courtesy of Robert H Edwards, U. of Cali, San Fran School of Medicine.
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Specification
Product Description
Mouse monoclonal antibody raised against native Lamp1.
Immunogen
Native purified Lamp1 from rat liver.
Host
Mouse
Reactivity
Hamster, Rat
Specificity
Detects an ~120KDa protein corresponding to the molecular mass of LAMP1 on SDS PAGE immunoblots.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining in transfected HeLa cells with Lamp1 monoclonal antibody, clone Ly1C6 (Cat # MAB6634).
Courtesy of Robert H Edwards, U. of Cali, San Fran School of Medicine.Immunoprecipitation
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Gene Info — Lamp1
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Publication Reference
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Tuberin is a component of lipid rafts and mediates caveolin-1 localization: role of TSC2 in post-Golgi transport.
Jones KA, Jiang X, Yamamoto Y, Yeung RS.
Experimental Cell Research 2004 May; 295(2):512.
Application：WB-Tr, Monkey, COS-1 cells.
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Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium.
Kannan K, Stewart RM, Bounds W, Carlsson SR, Fukuda M, Betzing KW, Holcombe RF.
Cellular Immunology 1996 Jul; 171(1):10.
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Glycoproteins of the lysosomal membrane.
Lewis V, Green SA, Marsh M, Vihko P, Helenius A, Mellman I.
The Journal of Cell Biology 1985 Jun; 100(6):1839.
Application：AFC, IF, Rat, NRK cells.
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Tuberin is a component of lipid rafts and mediates caveolin-1 localization: role of TSC2 in post-Golgi transport.
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