CaMKII monoclonal antibody, clone 22B1
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of mice ventricles with CaMKII monoclonal antibody, clone 22B1 (Cat # MAB6627).
Immunofluorescence
Immunofluorescence staining in mouse backskin- muscle and hair follicle with CaMKII monoclonal antibody, clone 22B1 (Cat # MAB6627).
Immunoelectron Microscopy
Immunoelectron microscopy from dissociated hippocampal neurons with CaMKII monoclonal antibody, clone 22B1 (Cat # MAB6627).
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of CaMKII.
Immunogen
A synthetic peptide corresponding to CaMKII.
Host
Mouse
Reactivity
Rat
Specificity
Detects phosphorylated CaMKII from rat tissues. This antibody is specific to alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta).
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mice ventricles with CaMKII monoclonal antibody, clone 22B1 (Cat # MAB6627).Immunofluorescence
Immunofluorescence staining in mouse backskin- muscle and hair follicle with CaMKII monoclonal antibody, clone 22B1 (Cat # MAB6627).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Immunoelectron Microscopy
Immunoelectron microscopy from dissociated hippocampal neurons with CaMKII monoclonal antibody, clone 22B1 (Cat # MAB6627). -
Publication Reference
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Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase.
Hughes K, Edin S, Antonsson A, Grundström T.
The Journal of Biological Chemistry 2001 Sep; 276(38):36008.
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Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation.
Makhinson M, Chotiner JK, Watson JB, O'Dell TJ.
The Journal of Neuroscience 1999 Apr; 19(7):2500.
Application：WB-Ti, Mouse, Hippocampal slices.
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Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation.
Barria A, Muller D, Derkach V, Griffith LC, Soderling TR.
Science 1997 Jun; 276(5321):2042.
Application：WB-Ti, Mouse, Brain.
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Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase.
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