Camk2a monoclonal antibody, clone 6G9
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More Files
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Immunofluorescence
Immunofluorescence staining in cultured rat cortical neurons with Camk2a monoclonal antibody, clone 6G9 (Cat # MAB6626).
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Specification
Product Description
Mouse monoclonal antibody raised against Camk2a.
Immunogen
Partially purified rat Camk2a.
Host
Mouse
Reactivity
Bovine, Mouse, Rat
Specificity
Detects ~50-60KDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (50% glycerol, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunofluorescence
Immunofluorescence staining in cultured rat cortical neurons with Camk2a monoclonal antibody, clone 6G9 (Cat # MAB6626).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
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Gene Info — Camk2a
Entrez GeneID
25400Gene Name
Camk2a
Gene Alias
PK2CDD, PKCCD
Gene Description
calcium/calmodulin-dependent protein kinase II alpha
Gene Ontology
HyperlinkOther Designations
Ca2+/calmodulin-dependent protein kinase II alpha|alpha CaM kinase II|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha subunit
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Publication Reference
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Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase.
Hughes K, Edin S, Antonsson A, Grundström T.
The Journal of Biological Chemistry 2001 Sep; 276(38):36008.
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Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation.
Makhinson M, Chotiner JK, Watson JB, O'Dell TJ.
The Journal of Neuroscience 1999 Apr; 19(7):2500.
Application：WB-Ti, Mouse, Hippocampal slices.
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Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation.
Barria A, Muller D, Derkach V, Griffith LC, Soderling TR.
Science 1997 Jun; 276(5321):2042.
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Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase.
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