HSPD1 monoclonal antibody, clone LK1
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More Files
- More Functions
Western Blot
Western blot analysis of cell and tissue lysates with HSPD1 monoclonal antibody, clone LK1 (Cat # MAB6621).
Immunohistochemistry
Immunohistochemical staining of human colon cancer with HSPD1 monoclonal antibody, clone LK1 (Cat # MAB6621). In 100,000 fold amplify.
Immunocytochemistry
Immunocytochemical staining of human skin fibroblast cells with HSPD1 monoclonal antibody, clone LK1 (Cat # MAB6621).
A : control.
B : Incubath with antibody after 24h later.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant HSPD1.
Immunogen
Recombinant protein corresponding to human HSPD1.
Host
Mouse
Reactivity
Bovine, Chicken, Dog, Frog, Fruit fly, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
Specificity
Detects an ~60KDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (0.05 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of cell and tissue lysates with HSPD1 monoclonal antibody, clone LK1 (Cat # MAB6621).Immunohistochemistry
Immunohistochemical staining of human colon cancer with HSPD1 monoclonal antibody, clone LK1 (Cat # MAB6621). In 100,000 fold amplify.Immunocytochemistry
Immunocytochemical staining of human skin fibroblast cells with HSPD1 monoclonal antibody, clone LK1 (Cat # MAB6621).
A : control.
B : Incubath with antibody after 24h later.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — HSPD1
Entrez GeneID
3329Gene Name
HSPD1
Gene Alias
CPN60, GROEL, HLD4, HSP60, HSP65, HuCHA60, SPG13
Gene Description
heat shock 60kDa protein 1 (chaperonin)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Two pseudogenes, both located on chromosome 8, have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq
Other Designations
P60 lymphocyte protein|chaperonin|heat shock 60kD protein 1 (chaperonin)|heat shock protein 65|mitochondrial heat shock 60kD protein 1 variant 1|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1|spastic paraplegia 13 (autosomal dominant)
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Interactome
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Pathway
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Disease
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Publication Reference
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Interaction of antibodies against cytomegalovirus with heat-shock protein 60 in pathogenesis of atherosclerosis.
Bason C, Corrocher R, Lunardi C, Puccetti P, Olivieri O, Girelli D, Navone R, Beri R, Millo E, Margonato A, Martinelli N, Puccetti A.
Lancet 2003 Dec; 362(9400):1971.
Application：IP, WB-Ce, WB-Tr, Flow Cyt, ELISA, Human, Monkey, Endothelial, COS cells, Serum.
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Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration.
Itoh H, Komatsuda A, Ohtani H, Wakui H, Imai H, Sawada K, Otaka M, Ogura M, Suzuki A, Hamada F.
European Journal of Biochemistry 2002 Dec; 269(23):5931.
Application：IHC, WB, Rat, Livers, Kidneys.
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The Hsp70 and Hsp60 chaperone machines.
Bukau B, Horwich AL.
Cell 1998 Feb; 92(3):351.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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Interaction of antibodies against cytomegalovirus with heat-shock protein 60 in pathogenesis of atherosclerosis.
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