G6PD monoclonal antibody, clone AT2F6
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Specification
Product Description
Mouse monoclonal antibody raised aganist partial recombinant G6PD.
Immunogen
Recombinant protein corresponding to amino acids 35-506 of human G6PD.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF7 cell lysate, Lane 2: HeLa cell lysate, Lane 3: Jurkat cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with G6PD monoclonal antibody, clone AT2F6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — G6PD
Entrez GeneID
2539Protein Accession#
NP_001035810.1Gene Name
G6PD
Gene Alias
G6PD1
Gene Description
glucose-6-phosphate dehydrogenase
Omim ID
305900Gene Ontology
HyperlinkGene Summary
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026034|glucose-6-phosphate 1-dehydrogenase|glucose-6-phosphate dehydrogenase, G6PD
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Interactome
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Pathway
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Disease
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Publication Reference
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Purification and detailed study of two clinically different human glucose 6-phosphate dehydrogenase variants, G6PD(Plymouth) and G6PD(Mahidol): Evidence for defective protein folding as the basis of disease.
Huang Y, Choi MY, Au SW, Au DM, Lam VM, Engel PC.
Molecular Genetics and Metabolism 2008 Jan; 93(1):44.
Application:WB, Bacteria, E. coli DF 213 cells.
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[Glucose-6-phosphate dehydrogenase (G6PD) deficiency--a cause of anaemia in pregnant women] [Article in Polish].
Kuliszkiewicz-Janus M, Zimny A.
Pol Arch Med Wewn 2003 Nov; 110(5):1327.
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Purification and detailed study of two clinically different human glucose 6-phosphate dehydrogenase variants, G6PD(Plymouth) and G6PD(Mahidol): Evidence for defective protein folding as the basis of disease.
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