Recombinant protein corresponding to amino acids 35-506 of human G6PD.
Host:
Mouse
Reactivity:
Human
Form:
Liquid
Purification:
Protein G purification
Isotype:
IgG2b, kappa
Recommend Usage:
ELISA Flow Cytometry Immunohistochemistry Immunocytochemistry Immunofluorescence Western Blot The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of Lane 1: MCF7 cell lysate, Lane 2: HeLa cell lysate, Lane 3: Jurkat cell lysate.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of HeLa cells. The cell was stained with G6PD monoclonal antibody, clone AT2F6 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq