Mouse monoclonal antibody raised against partial recombinant SP1.
Recombinant protein corresponding to amino acids 150-200 of human SP1.
Theoretical MW (kDa):
This affinity purified antibody recognizes ~90 KDa of human SP1.
Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:50) Flow Cytometry (5-10 ug/mL) The optimal working dilution should be determined by the end user.
In TBS, pH 7.2 (BSA, 10% Proclin300)
Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Human tonsil tissue stained with SP1 monoclonal antibody, clone 15H09 (Cat # MAB6565) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.