CD68 monoclonal antibody, clone SPM281
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human tonsil tissue stained with CD68 monoclonal antibody, clone SPM281 (Cat # MAB6564) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Mouse monoclonal antibody raised against CD68.
Immunogen
Native from subcellular fraction of human alveolar macrophages.
Host
Mouse
Theoretical MW (kDa)
110
Reactivity
Human, Monkey, Mouse, Rat
Specificity
This affinity purified antibody recognizes ~110 KDa of human CD68.
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human tonsil tissue stained with CD68 monoclonal antibody, clone SPM281 (Cat # MAB6564) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD68
Entrez GeneID
968Gene Name
CD68
Gene Alias
DKFZp686M18236, GP110, SCARD1
Gene Description
CD68 molecule
Omim ID
153634Gene Ontology
HyperlinkGene Summary
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq
Other Designations
CD68 antigen|OTTHUMP00000135285|macrophage antigen CD68|macrosialin|scavenger receptor class D, member 1
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Interactome
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Pathway
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Publication Reference
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Accelerated accumulation of retinal α-synuclein (pSer129) and tau, neuroinflammation and autophagic dysregulation in a seeded mouse model of Parkinson's disease.
Mammadova N, Summers CM, Kokemuller RD, He Q, Ding S, Baron T, Yu C, Valentine RJ, Sakaguchi DS, Kanthasamy AG, Greenlee JJ, Heather West Greenlee M.
Neurobiology of Disease 2019 Jan; 121:1.
Application：IF, IHC-P, Mouse, Mouse retina.
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Evolutional Characterization of Photochemically Induced Stroke in Rats: a Multimodality Imaging and Molecular Biological Study.
Liu NW, Ke CC, Zhao Y, Chen YA, Chan KC, Tan DT, Lee JS, Chen YY, Hsu TW, Hsieh YJ, Chang CW, Yang BH, Huang WS, Liu RS.
Translational Stroke Research 2016 Dec; 8(3):244.
Application：IHC-P, Rat, Rat brain.
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Combination of cadmium and high cholesterol levels as a risk factor for heart fibrosis.
Turkcan A, Scharinger B, Grabmann G, Keppler BK, Laufer G, Bernhard D, Messner B.
Toxicological Sciences 2015 Jun; 145(2):360.
Application：IF, Mouse, Hearts.
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Accelerated accumulation of retinal α-synuclein (pSer129) and tau, neuroinflammation and autophagic dysregulation in a seeded mouse model of Parkinson's disease.
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