TDP1 monoclonal antibody, clone AT1F2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MCF-7 (30 ug) cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with TDP1 monoclonal antibody, clone AT1F2 (Cat # MAB6511) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with TDP1 monoclonal antibody, clone AT1F2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of Jurkat cell line, staining at 2-5 ug for x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised aganist partial recombinant TDP1.
Immunogen
Recombinant protein corresponding to amino acids 1-298 of human TDP1.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 (30 ug) cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with TDP1 monoclonal antibody, clone AT1F2 (Cat # MAB6511) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of Hep3B cells. The cell was stained with TDP1 monoclonal antibody, clone AT1F2 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of Jurkat cell line, staining at 2-5 ug for x106cells. (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — TDP1
Entrez GeneID
55775Protein Accession#
NP_060789Gene Name
TDP1
Gene Alias
FLJ11090, MGC104252
Gene Description
tyrosyl-DNA phosphodiesterase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Tyrosyl-DNA phosphodiesterase as a target for anticancer therapy.
Dexheimer TS, Antony S, Marchand C, Pommier Y.
Anti-Cancer Agents in Medicinal Chemistry 2008 May; 8(4):381.
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TDP1-dependent DNA single-strand break repair and neurodegeneration.
El-Khamisy SF, Caldecott KW.
Mutagenesis 2006 Jul; 21(4):219.
Application：WB, Human, Mammalian cells .
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Tyrosyl-DNA phosphodiesterase as a target for anticancer therapy.
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