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Last updated: 2021/1/17
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TBCA monoclonal antibody, clone AT1A5 

  • Catalog # : MAB6509
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised aganist full length recombinant TBCA.
  • Immunogen:
  • Recombinant protein corresponding to full length human TBCA.
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Isotype:
  • IgG2b, kappa
  • Recommend Usage:
  • ELISA
    Flow Cytometry
    Immunocytochemistry
    Immunofluorescence
    Western Blot
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of LNCaP (20 ug) cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with TBCA monoclonal antibody, clone AT1A5 (Cat # MAB6509) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
  • Immunocytochemistry
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis A549 cells. The cell was stained with TBCA monoclonal antibody, clone AT1A5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x 106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunocytochemistry
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 6902
  • Gene Name:
  • TBCA
  • Gene Alias:
  • -
  • Gene Description:
  • tubulin folding cofactor A
  • Gene Summary:
  • The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. [provided by RefSeq
  • Other Designations:
  • chaperonin cofactor A,tubulin-specific chaperone a
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