Cell lysates of LNCaP (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with TBCA monoclonal antibody, clone AT1A5 (Cat # MAB6509) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. [provided by RefSeq
chaperonin cofactor A,tubulin-specific chaperone a