Western blot analysis of LNCaP (20 ug) cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with TBCA monoclonal antibody, clone AT1A5 (Cat # MAB6509) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis A549 cells. The cell was stained with TBCA monoclonal antibody, clone AT1A5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x 106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. [provided by RefSeq
Other Designations:
chaperonin cofactor A,tubulin-specific chaperone a