TBCA monoclonal antibody, clone AT1A5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of LNCaP (20 ug) cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with TBCA monoclonal antibody, clone AT1A5 (Cat # MAB6509) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence
Immunofluorescence analysis A549 cells. The cell was stained with TBCA monoclonal antibody, clone AT1A5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry
Flow cytometric analysis of HeLa cell line, staining at 2-5 ug for 1x 106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
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Specification
Product Description
Mouse monoclonal antibody raised aganist full length recombinant TBCA.
Immunogen
Recombinant protein corresponding to full length human TBCA.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG2b, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LNCaP (20 ug) cell lysate were resolved by SDS-PAGE, transferred to NC membrane and probed with TBCA monoclonal antibody, clone AT1A5 (Cat # MAB6509) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis A549 cells. The cell was stained with TBCA monoclonal antibody, clone AT1A5 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HeLa cell line, staining at 2-5 ug for 1x 106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. -
Gene Info — TBCA
Entrez GeneID
6902Protein Accession#
NP_004598Gene Name
TBCA
Gene Alias
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Gene Description
tubulin folding cofactor A
Omim ID
610058Gene Ontology
HyperlinkGene Summary
The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. [provided by RefSeq
Other Designations
chaperonin cofactor A|tubulin-specific chaperone a
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Interactome
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Disease
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Publication Reference
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Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death.
Nolasco S, Bellido J, Goncalves J, Zabala JC, Soares H.
FEBS Letters 2005 Jul; 579(17):3515.
Application：WB-Tr, Human, HeLa, MCF-7 cells.
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Pathway leading to correctly folded beta-tubulin.
Tian G, Huang Y, Rommelaere H, Vandekerckhove J, Ampe C, Cowan NJ.
Cell 1996 Jul; 86(2):287.
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Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death.
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