RNH1 monoclonal antibody, clone AT1H23
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Specifications
Product Description
Mouse monoclonal antibody raised aganist partial recombinant RNH1.
Immunogen
Recombinant protein corresponding to amino acids 7-461 of human RNH1.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Jurkat cell lysate, Lane 2: HepG2 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of PC3 cells. The cell was stained with RNH1 monoclonal antibody, clone AT1H23 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RNH1
Entrez GeneID
6050Protein Accession#
NP_002930Gene Name
RNH1
Gene Alias
MGC18200, MGC4569, MGC54054, RAI, RNH
Gene Description
ribonuclease/angiogenin inhibitor 1
Omim ID
173320Gene Ontology
HyperlinkOther Designations
OTTHUMP00000147628|Placental ribonuclease inhibitor|ribonuclease/angiogenin inhibitor
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Interactomes
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Diseases
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Publication Reference
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Functional expression of recombinant human ribonuclease/angiogenin inhibitor in stably transformed Drosophila melanogaster S2 cells.
Park JH, Hwang IS, Kim KI, Lee JM, Park YM, Park CH, Chung IS.
Cytotechnology 2008 May; 57(1):93.
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Inhibition of human pancreatic ribonuclease by the human ribonuclease inhibitor protein.
Johnson RJ, McCoy JG, Bingman CA, Phillips GN Jr, Raines RT.
Journal of Molecular Biology 2007 Apr; 368(2):434.
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Anti-tumor effect of hematopoietic cells carrying the gene of ribonuclease inhibitor.
Fu P, Chen J, Tian Y, Watkins T, Cui X, Zhao B.
Cancer Gene Therapy 2005 Mar; 12(3):268.
Application:IF, WB-Ti, Mouse, Bone marrow, Spleen, Liver.
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Functional expression of recombinant human ribonuclease/angiogenin inhibitor in stably transformed Drosophila melanogaster S2 cells.
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