Cd19 monoclonal antibody, clone 1D3 (PE)
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Specification
Product Description
Rat monoclonal antibody raised against native Cd19.
Immunogen
Native purified from mouse Cd19 transfected cell line.
Host
Rat
Theoretical MW (kDa)
95
Reactivity
Mouse
Specificity
This antibody detects mouse CD19, 95 KDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
Form
Liquid
Conjugation
PE
Concentration
0.1 mg/mL
Isotype
IgG2a
Recommend Usage
Flow Cytometry (1 ug/mL)
Functional Study
Immunohistochemistry
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store at 4°C.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Functional Study
Flow Cytometry
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Gene Info — Cd19
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Publication Reference
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The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment.
Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S.
The Journal of Immunology 2003 Oct; 171(8):4062.
Application:Flow Cyt, IP, Mouse, B cells.
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Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement.
Inabe K, Kurosaki T.
Blood 2002 Jan; 99(2):584.
Application:Flow Cyt, IP, WB-Tr, Chicken, Mouse, B cells, DT40 cells.
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The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens.
Cherukuri A, Cheng PC, Pierce SK.
J Immunol 2001 Jul; 167(1):163.
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The signaling activity of murine CD19 is regulated during cell development.
Krop I, Shaffer AL, Fearon DT, Schlissel MS.
Journal of Immunology 1996 Jul; 157(1):48.
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Self-renewal of B-1 lymphocytes is dependent on CD19.
Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT.
European Journal of Immunology 1996 Jan; 26(1):238.
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The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment.
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