CD34 monoclonal antibody, clone QBEnd-10 (PE)
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Flow Cytometry
Flow cytometric analysis (surface staining) of CD34 in human peripheral blood.
-
Specification
Product Description
Mouse monoclonal antibody raised against native CD34.
Immunogen
Native purified CD34 from human endothelial vesicles.
Host
Mouse
Theoretical MW (kDa)
110-115
Reactivity
Human, Non-Human Primates
Specificity
This antibody reacts with Class II epitope on CD34 (Mucosialin), a 110-115 KDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitorscells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
Form
Liquid
Conjugation
PE
Preparation Method
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Isotype
IgG1
Recommend Usage
Flow Cytometry (The reagent is designed for analysis of human blood cells using 20 uL reagent/100 uL of whole blood or 106 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (15 mM sodium azide).
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Flow Cytometry
Flow cytometric analysis (surface staining) of CD34 in human peripheral blood. -
Gene Info — CD34
Entrez GeneID
947Gene Name
CD34
Gene Alias
-
Gene Description
CD34 molecule
Omim ID
142230Gene Ontology
HyperlinkGene Summary
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM
Other Designations
CD34 antigen|OTTHUMP00000034733|OTTHUMP00000034734|hematopoietic progenitor cell antigen CD34
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Simian immunodeficiency virus infection and flow cytometric characterization of Japanese macaque (Macaca fuscata) hematopoietic cells.
Tsukamoto T, Nakamura K, Okada S.
Journal of Medical Primatology 2020 Apr; 49(2):116.
Application：Flow Cyt, Monkey, Bone marrow cells, PBMCs.
-
Streamlined production of genetically modified T cells with activation, transduction and expansion in closed-system G-Rex bioreactors.
Gagliardi C, Khalil M, Foster AE.
Cytotherapy 2019 Dec; 21(12):1246.
Application：Flow Cyt, Human, PBMCs.
-
Onset of Appearance and Potential Significance of Telocytes in the Developing Fetal Lung.
Awad M, Gaber W, Ibrahim D.
Microscopy and Microanalysis 2019 Oct; 25(5):1246.
Application：IHC-P, Rabbit, Lung.
-
Two-Dimensional Regulation of CAR-T Cell Therapy with Orthogonal Switches.
Duong MT, Collinson-Pautz MR, Morschl E, Lu A, Szymanski SP, Zhang M, Brandt ME, Chang WC, Sharp KL, Toler SM, Slawin KM, Foster AE, Spencer DM, Bayle JH.
Molecular Therapy Oncolytics 2018 Dec; 12:124.
Application：Flow Cyt, Human, PBMCs.
-
Constitutively active MyD88/CD40 costimulation enhances expansion and efficacy of chimeric antigen receptor T cells targeting hematological malignancies.
Collinson-Pautz MR, Chang WC, Lu A, Khalil M, Crisostomo JW, Lin PY, Mahendravada A, Shinners NP, Brandt ME, Zhang M, Duong M, Bayle JH, Slawin KM, Spencer DM, Foster AE.
Leukemia 2019 Feb; [Epub].
Application：Flow Cyt, Human, Gene-modified T cells.
-
Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way.
Traore Y, Hirn J.
European Journal of Immunology 1994 Oct; 24(10):2304.
Application：Flow Cyt, Func, Human, KG-l cells.
-
QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors.
Poblet E, Jimenez-Acosta F, Rocamora A.
Journal of Cutaneous Pathology 1994 Jun; 21(3):224.
Application：IHC-P, Human, Human follicular tumors.
-
Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond.
Grimsley PG, Amos TA, Gordon MY, Greaves MF.
Leukemia 1993 Jun; 7(6):898.
-
Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells.
Sutherland DR, Marsh JC, Davidson J, Baker MA, Keating A, Mellors A.
Exp Hematol 1992 Jun; 20(5):590.
-
Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours.
Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY.
Journal of Clinical Pathology 1992 Feb; 45(2):143.
-
Simian immunodeficiency virus infection and flow cytometric characterization of Japanese macaque (Macaca fuscata) hematopoietic cells.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com