BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells. Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures.
Theoretical MW (kDa):
This antibody binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 KDa membrane-associated protein accelerating bidirectional movement of plasmamembrane phospholipids during conditions of elevated calcium.
The optimal working dilution should be determined by the end user.
In ascites (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunofluorescence staining of Plscr1 in rat basophilic leukemia (RBL) cell line using Plscr1 monoclonal antibody, clone 13A6 [TEC-23] (Cat # MAB6482).
Detection of Plscr1 in rat basophilic leukemia (RBL) cell line lysate (Lane 1) and in Plscr1 immunoprecipitate from RBL lysate (Lane 2). Plscr1 monoclonal antibody, clone 13A6 [TEC-23] (Cat # MAB6482) was used both for immunoprecipitation and immunodetection.