Plscr1 monoclonal antibody, clone 13A6 [TEC-23]
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More Files
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Immunofluorescence
Immunofluorescence staining of Plscr1 in rat basophilic leukemia (RBL) cell line using Plscr1 monoclonal antibody, clone 13A6 [TEC-23] (Cat # MAB6482).
Immunoprecipitation
Detection of Plscr1 in rat basophilic leukemia (RBL) cell line lysate (Lane 1) and in Plscr1 immunoprecipitate from RBL lysate (Lane 2). Plscr1 monoclonal antibody, clone 13A6 [TEC-23] (Cat # MAB6482) was used both for immunoprecipitation and immunodetection.
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Specification
Product Description
Mouse monoclonal antibody raised against Plscr1.
Immunogen
BALB/c mice were immunized with pooled lipid rafts isolated from RBL-2H3 cells. Hybridoma cells were obtained after fusion of SP02 mouse myeloma cells with spleen cells of immunized mice using standard procedures.
Host
Mouse
Theoretical MW (kDa)
37-49
Reactivity
Rat
Specificity
This antibody binds to rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 KDa membrane-associated protein accelerating bidirectional movement of plasmamembrane phospholipids during conditions of elevated calcium.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In ascites (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of Plscr1 in rat basophilic leukemia (RBL) cell line using Plscr1 monoclonal antibody, clone 13A6 [TEC-23] (Cat # MAB6482).Immunoprecipitation
Detection of Plscr1 in rat basophilic leukemia (RBL) cell line lysate (Lane 1) and in Plscr1 immunoprecipitate from RBL lysate (Lane 2). Plscr1 monoclonal antibody, clone 13A6 [TEC-23] (Cat # MAB6482) was used both for immunoprecipitation and immunodetection.Flow Cytometry
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Gene Info — Plscr1
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Publication Reference
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Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis.
Smrz D, Lebduska P, Draberova L, Korb J, Draber P.
The Journal of Biological Chemistry 2008 Apr; 283(16):10904.
Application：IF, Rat, Rat basophilic leukemia cells.
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Phospholipid scramblase 1 potentiates the antiviral activity of interferon.
Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH.
Journal of Virology 2004 Sep; 78(17):8983.
Application：WB-Tr, Human, Hey1B cells.
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Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1.
Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ.
Cancer Research 2002 Jan; 62(2):397.
Application：IF, IHC-Fr, WB-Tr, Human, HEY1B cells.
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Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis.
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