Neurofilament heavy protein monoclonal antibody, clone NF-05
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More Files
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Western Blot (Tissue lysate)
Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by Neurofilament heavy protein monoclonal antibody, clone NF-05 (Cat # MAB6481).
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Specification
Product Description
Mouse monoclonal antibody raised against native Neurofilament heavy protein.
Immunogen
Native purified from pig brain neurofilament protein-enriched fraction after depolymerization of microtubules.
Host
Mouse
Theoretical MW (kDa)
210
Reactivity
Human, Pig, Rat
Specificity
This antibody recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 KDa intracellular structural protein of Intermediate Filament Proteins family.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
ELISA
Immunohistochemistry
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blotting analysis of neurofilament heavy protein in porcine brain lysate (reducing conditions) by Neurofilament heavy protein monoclonal antibody, clone NF-05 (Cat # MAB6481).Immunohistochemistry (Frozen sections)
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia.
Petzold A, Keir G, Warren J, Fox N, Rossor MN.
Neuro-Degenerative Diseases 2007 Jun; 4(2-3):185.
Application：ELISA, Human, Cerebrospinal fluid from patients with neurodegenerative dementias.
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High CSF neurofilament heavy chain levels in neuromyelitis optica.
Miyazawa I, Nakashima I, Petzold A, Fujihara K, Sato S, Itoyama Y.
Neurology. 2007 Mar; 68(11):865.
Application：ELISA, Human, CSF from patients with Neuromyelitis optica, multiple sclerosis.
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Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex.
Porchet R, Probst A, Draberova E, Draber P, Riederer IM, Riederer BM.
Neuroreport 2003 May; 14(7):929.
Application：IF, IHC, Human, Human entorhinal cortex.
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A systematic review and meta-analysis of CSF neurofilament protein levels as biomarkers in dementia.
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