LST1 monoclonal antibody, clone LST1/02
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blotting analysis (non-reducing conditions) of LST1 in whole cell lysate of U-937 (human Caucasian histiocytic lymphoma cell line).
Lane 1 : Immunostaining with Isotype mouse IgG1 control.
Lane 2-3 : Immunostaining with LST1 monoclonal antibody, clone LST1/02 (Cat # MAB6480), two independent batches.
Note : It migrates on SDS PAGE gels as approximately 25-28 KDa molecule.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of LST1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to human LST1.
Sequence
SSEGPDLRGRDKRGT
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with an extracellular epitope of LST1, an approximately 6-11 KDa protein expressed as various transmembrane or soluble forms. It migrates on SDS PAGE gels asapproximately 25-28 KDa molecule.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blotting analysis (non-reducing conditions) of LST1 in whole cell lysate of U-937 (human Caucasian histiocytic lymphoma cell line).
Lane 1 : Immunostaining with Isotype mouse IgG1 control.
Lane 2-3 : Immunostaining with LST1 monoclonal antibody, clone LST1/02 (Cat # MAB6480), two independent batches.
Note : It migrates on SDS PAGE gels as approximately 25-28 KDa molecule.Immunocytochemistry
Immunoprecipitation
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Gene Info — LST1
Entrez GeneID
7940Gene Name
LST1
Gene Alias
B144, D6S49E, LST-1, MGC119006, MGC119007
Gene Description
leukocyte specific transcript 1
Omim ID
109170Gene Ontology
HyperlinkOther Designations
OTTHUMP00000038208|OTTHUMP00000038211|OTTHUMP00000038213|OTTHUMP00000038312|OTTHUMP00000038314|OTTHUMP00000038315|OTTHUMP00000038651|OTTHUMP00000038655|OTTHUMP00000038657|leucocyte specific transcript 1|lymphocyte antigen 117
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Interactome
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Disease
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Publication Reference
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LST1 and NCR3 expression in autoimmune inflammation and in response to IFN-gamma, LPS and microbial infection.
Mulcahy H, O'Rourke KP, Adams C, Molloy MG, O'Gara F.
Immunogenetics 2006 Jan; 57(12):893.
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Functional analysis of B144/LST1: a gene in the tumor necrosis factor cluster that induces formation of long filopodia in eukaryotic cells.
Raghunathan A, Sivakamasundari R, Wolenski J, Poddar R, Weissman SM.
Experimental Cell Research 2001 Aug; 268(2):230.
Application：IF, IHC-Fr, WB, Human, Mouse, Dendritic cells, Spleen, Thymus.
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Characterization of the promoter of human leukocyte-specific transcript 1. A small gene with a complex pattern of alternative transcripts.
Yu X, Weissman SM.
The Journal of Biological Chemistry 2000 Nov; 275(44):34597.
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LST1 and NCR3 expression in autoimmune inflammation and in response to IFN-gamma, LPS and microbial infection.
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