FOLH1 monoclonal antibody, clone GCP-05 (PE)
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More Files
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Flow Cytometry
Flow cytometric analysis of FOLH1 using FOLH1 monoclonal antibody, clone GCP-05 (Cat # MAB3611) and FOLH1 monoclonal antibody, clone GCP-05 (PE) (Cat # MAB6477) on LNCaP cell line (positive, green) and HeLa cells (negative, blue).
Flow Cytometry
Flow cytometric analysis of FOLH1 by various monoclonal antibodies (GCP-01, GCP-02, GCP-04, GCP-05) on LNCaP cell line.
Legend :
green column - 1 ug/mL , LNCaP cell line.
blue column - 5 ug/mL , LNCaP cell line.
yellow column - negative cell line.
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Specification
Product Description
Mouse monoclonal antibody raised against FOLH1.
Immunogen
Amino acids 44-750 of human FOLH1.
Host
Mouse
Reactivity
Human
Specificity
This antibody ecognizes extracellular domain of glutamate carboxy peptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 KDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is animportant prostate tumour marker.
Form
Liquid
Conjugation
PE
Concentration
0.1 mg/mL
Isotype
IgG1
Recommend Usage
Flow Cytometry (1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Flow Cytometry
Flow cytometric analysis of FOLH1 using FOLH1 monoclonal antibody, clone GCP-05 (Cat # MAB3611) and FOLH1 monoclonal antibody, clone GCP-05 (PE) (Cat # MAB6477) on LNCaP cell line (positive, green) and HeLa cells (negative, blue).Flow Cytometry
Flow cytometric analysis of FOLH1 by various monoclonal antibodies (GCP-01, GCP-02, GCP-04, GCP-05) on LNCaP cell line.
Legend :
green column - 1 ug/mL , LNCaP cell line.
blue column - 5 ug/mL , LNCaP cell line.
yellow column - negative cell line. -
Gene Info — FOLH1
Entrez GeneID
2346Gene Name
FOLH1
Gene Alias
FGCP, FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA, mGCP
Gene Description
folate hydrolase (prostate-specific membrane antigen) 1
Omim ID
600934Gene Ontology
HyperlinkGene Summary
This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants. [provided by RefSeq
Other Designations
N-acetylated alpha-linked acidic dipeptidase 1|cell growth-inhibiting protein 27|folate hydrolase 1|folylpoly-gamma-glutamate carboxypeptidase|glutamate carboxylase II|glutamate carboxypeptidase II|membrane glutamate carboxypeptidase|prostate specific mem
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Interactome
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Disease
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Publication Reference
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Expression of glutamate carboxypeptidase II in human brain.
Sacha P, Zamecnik J, Barinka C, Hlouchova K, Vicha A, Mlcochova P, Hilgert I, Eckschlager T, Konvalinka J.
Neuroscience 2007 Feb; 144(4):1361.
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Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity.
Barinka C, Sacha P, Sklenar J, Man P, Bezouska K, Slusher BS, Konvalinka J.
Protein Science 2004 Jun; 13(6):1627.
Application：WB-Tr, Insect, S2 cells.
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Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding.
Barinka C, Mlcochova P, Sacha P, Hilgert I, Majer P, Slusher BS, Horejsi V, Konvalinka J.
European Journal of Biochemistry 2004 Jul; 271(13):2782.
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Expression of glutamate carboxypeptidase II in human brain.
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