Mouse monoclonal antibody raised against recombinant PRNP.
Recombinant protein corresponding to human PRNP.
This antibody recognizes human prion protein (PrP). Diglycosylated form of PrP has ~40 KDa, monoglycosylated form ~30 KDa, and nonglycosylated form ~19-21 KDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prionprotein.
Western Blot (0.5 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4 (0.09% sodium azide)
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blotting analysis of Creutzfeld-Jakob disease (CJD) negative (Lane 1, 2) and CJD positive (Lane 3, 4) human brain material using PRNP monoclonal antibody, clone EM-20 (Cat # MAB6472). CJD positive patient has proteinase K resistent prion protein. Lane 1, 4 : Samples with proteinase K treatment Lane 2, 3 : Samples without proteinase K treatment.
The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that tends to aggregate into rod-like structures. The encoded protein contains a highly unstable region of five tandem octapeptide repeats. This gene is found on chromosome 20, approximately 20 kbp upstream of a gene which encodes a biochemically and structurally similar protein to the one encoded by this gene. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
CD230 antigen,OTTHUMP00000030162,OTTHUMP00000030163,major prion protein,p27-30,prion protein PrP,prion-related protein