Ptprc monoclonal antibody, clone EM-05
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More Files
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Flow Cytometry
Surface staining of Ptprc knock-out cells with Ptprc monoclonal antibody, clone EM-05 (Cat # MAB6467).
Flow Cytometry
Surface staining of mouse blood lymphocytes with Ptprc monoclonal antibody, clone EM-05 (APC).
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Specification
Product Description
Rat monoclonal antibody raised against native Ptprc.
Immunogen
Native purified Ptprc from mouse peripheral blood leukocytes.
Host
Rat
Reactivity
Mouse
Specificity
This antibody reacts with mouse CD45 antigen (Leukocyte Common Antigen), asingle chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
Form
Liquid
Isotype
IgG
Recommend Usage
Immunocytochemistry
Immunoprecipitation
Flow Cytometry (2 ug/mL)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunoprecipitation
Flow Cytometry
Surface staining of Ptprc knock-out cells with Ptprc monoclonal antibody, clone EM-05 (Cat # MAB6467).Flow Cytometry
Surface staining of mouse blood lymphocytes with Ptprc monoclonal antibody, clone EM-05 (APC). -
Gene Info — Ptprc
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Interactome
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Publication Reference
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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.
Desharnais P, Dupere-Minier G, Hamelin C, Devine P, Bernier J.
Apoptosis 2008 Feb; 13(2):197.
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Combinations of CD45 isoforms are crucial for immune function and disease.
Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ.
Journal of Immunology 2006 Mar; 176(6):3417.
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CD45 isoform RB as a molecular target to oppose lipopolysaccharide-induced microglial activation in mice.
Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J.
Neuroscience Letters 2004 May; 362(1):26.
Application：Flow Cyt, Mouse , Mouse cultured microglial cells.
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Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents.
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