IgG (Fc) monoclonal antibody, clone EM-07
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More Files
- More Functions
Western Blot (Tissue lysate)
IgG, kappa light chain (Lane 1), IgG lambda light chain (Lane 2) and IgG Fc fragment (Lane 3) purified from human serum were analysed by Western blotting with Kappa light chains monoclonal antibody, clone MEM-09 (Cat # MAB6445) (Panel A) and IgG (Fc) monoclonal antibody, clone EM-07 (Cat # MAB6465) (Panel B).
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Specification
Product Description
Mouse monoclonal antibody raised against IgG (Fc).
Immunogen
Fusion protein of human IgG Fc fragment.
Host
Mouse
Reactivity
Human
Specificity
This antibody reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Form
Liquid
Isotype
IgG1
Recommend Usage
Flow Cytometry (1-4 ug/mL)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
IgG, kappa light chain (Lane 1), IgG lambda light chain (Lane 2) and IgG Fc fragment (Lane 3) purified from human serum were analysed by Western blotting with Kappa light chains monoclonal antibody, clone MEM-09 (Cat # MAB6445) (Panel A) and IgG (Fc) monoclonal antibody, clone EM-07 (Cat # MAB6465) (Panel B).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors.
Brinkmann V, Heusser CH.
Cellular Immunology 1993 Dec; 152(2):323.
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Serological abnormalities in juvenile chronic arthritis: a review of 46 cases.
Balogh Z, Meretey K, Falus A, Bozsoky S.
Annals of the Rheumatic Diseases 1980 Apr; 39(2):129.
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Serum immunoglobulins after surgical operation.
Fuller JM, Keyser JW.
Clinical Chemistry 1975 May; 21(6):667.
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T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors.
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