AGR2 and AGR3 monoclonal antibody, clone AGR3.4
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More Files
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Western Blot (Cell lysate)
Western blotting analysis of AGR3 protein by AGR2 and AGR3 monoclonal antibody, clone AGR3.4 (Cat # MAB6454) in H1229 (human lung carcinoma cell line, lane 1) compared to T-47D (human breast cancer cell line, lane 2).
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Specification
Product Description
Mouse monoclonal antibody raised against AGR2 and AGR3.
Immunogen
Purified human AGR2 and AGR3 protein.
Host
Mouse
Theoretical MW (kDa)
19-20
Reactivity
Human
Specificity
This antibody recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 KDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis.
Form
Liquid
Isotype
IgG1
Recommend Usage
Immunocytochemistry (1 ug/mL)
Immunohistochemistry (5 ug/mL)
Western Blot (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blotting analysis of AGR3 protein by AGR2 and AGR3 monoclonal antibody, clone AGR3.4 (Cat # MAB6454) in H1229 (human lung carcinoma cell line, lane 1) compared to T-47D (human breast cancer cell line, lane 2).Immunohistochemistry
Immunocytochemistry
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Publication Reference
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Anterior gradient 2 is expressed and secreted during the development of pancreatic cancer and promotes cancer cell survival.
Ramachandran V, Arumugam T, Wang H, Logsdon CD.
Cancer Research 2008 Oct; 68(19):7811.
Application：IHC-P, WB, Human, Aspc-1, BxPC3, CFPAC-1, HPAC, MiaPaCa-2, Panc-1, SU.86.86 cells, Human pancreatic cancer.
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Diversity of the protein disulfide isomerase family: identification of breast tumor induced Hag2 and Hag3 as novel members of the protein family.
Persson S, Rosenquist M, Knoblach B, Khosravi-Far R, Sommarin M, Michalak M.
Molecular Phylogenetics and Evolution 2005 Sep; 36(3):734.
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hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan.
Fletcher GC, Patel S, Tyson K, Adam PJ, Schenker M, Loader JA, Daviet L, Legrain P, Parekh R, Harris AL, Terrett JA.
British Journal of Cancer 2003 Feb; 88(4):579.
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Anterior gradient 2 is expressed and secreted during the development of pancreatic cancer and promotes cancer cell survival.
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