Tnfrsf18 monoclonal antibody, clone DTA-1
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Specification
Product Description
Rat monoclonal antibody raised against native Tnfrsf18.
Immunogen
Native purified Tnfrsf18 from mouse CD25+ CD4+ T cell line.
Host
Rat
Reactivity
Mouse
Form
Liquid
Isotype
IgG2b, lambda
Recommend Usage
Flow Cytometry (0.1-0.5 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Flow analysis of Tnfrsf18 in C57 mouse splenocytes using 0.1 ug of Tnfrsf18 monoclonal antibody, clone DTA-1 (Cat #MAB6244). Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-GITR antibody. -
Gene Info — Tnfrsf18
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Publication Reference
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GITR ligation blocks allograft protection by induced CD25+CD4+ regulatory T cells without enhancing effector T-cell function.
Bushell A, Wood K.
American Journal of Transplantation 2007 Apr; 7(4):759.
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Glucocorticoid-induced TNF receptor, a costimulatory receptor on naive and activated T cells, uses TNF receptor-associated factor 2 in a novel fashion as an inhibitor of NF-kappa B activation.
Esparza EM, Arch RH.
Journal of Immunology 2005 Jun; 174(12):7875.
Application:WB-Ce, Human, HEK Human embryonic kidney cell line.
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Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.
Kwon B, Yu KY, Ni J, Yu GL, Jang IK, Kim YJ, Xing L, Liu D, Wang SX, Kwon BS.
The Journal of Biological Chemistry 1999 Mar; 274(10):6056.
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GITR ligation blocks allograft protection by induced CD25+CD4+ regulatory T cells without enhancing effector T-cell function.
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