CASP7 monoclonal antibody, clone 25B881.1
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of CASP7 in A) human brain, B) mouse brain and C) rat brain lysate using CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) at 1 ug/mL .
Western Blot (Cell lysate)
Western blot analysis of CASP7 in Jurkat cells using CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) at 1 ug/mL. Cells were treated with 2 uM staurosporine for different time periods. CASP7 activation is detected in western blots by the presence of CASP7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) CASP7, depending on the treatment time points. A basal level of endogenous intermediate CASP7 can be see in untreated Jurkat cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of CASP7 in formalin-fixed, paraffin-embedded human cervix tumor tissue using an isotype control (top left) and CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) (bottom left, right) at 5 ug/mL .
-
Specification
Product Description
Mouse monoclonal antibody raised against recombinant CASP7.
Immunogen
Recombinant protein corresponding to human CASP7.
Host
Mouse
Reactivity
Human, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
Western blot analysis of CASP7 in A) human brain, B) mouse brain and C) rat brain lysate using CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) at 1 ug/mL .Western Blot (Cell lysate)
Western blot analysis of CASP7 in Jurkat cells using CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) at 1 ug/mL. Cells were treated with 2 uM staurosporine for different time periods. CASP7 activation is detected in western blots by the presence of CASP7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) CASP7, depending on the treatment time points. A basal level of endogenous intermediate CASP7 can be see in untreated Jurkat cells.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of CASP7 in formalin-fixed, paraffin-embedded human cervix tumor tissue using an isotype control (top left) and CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) (bottom left, right) at 5 ug/mL . -
Gene Info — CASP7
Entrez GeneID
840Protein Accession#
NP_001081408Gene Name
CASP7
Gene Alias
CMH-1, ICE-LAP3, MCH3
Gene Description
caspase 7, apoptosis-related cysteine peptidase
Omim ID
601761Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq
Other Designations
ICE-like apoptotic protease 3|Lice2 alpha/beta/gamma|OTTHUMP00000020510|OTTHUMP00000020511|OTTHUMP00000020512|OTTHUMP00000020514|apoptotic protease MCH-3|caspase 7|caspase 7 isoform delta|caspase 7, apoptosis-related cysteine protease
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis.
Yu CC, Li CF, Chen IH, Lai MT, Lin ZJ, Korla PK, Chai CY, Ko G, Chen CM, Hwang T, Lee SC, Sheu JJ.
The Journal of Pathology 2019 Aug; 248(4):476.
Application：WB-Tr, Human, RT4 cells.
-
Caspases: the executioners of apoptosis.
Cohen GM.
The Biochemical Journal 1997 Aug; 326 ( Pt 1):1.
-
Mch3, a novel human apoptotic cysteine protease highly related to CPP32.
Fernandes-Alnemri T, Takahashi A, Armstrong R, Krebs J, Fritz L, Tomaselli KJ, Wang L, Yu Z, Croce CM, Salveson G, et al..
Cancer Research 1995 Dec; 55(24):6045.
-
YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com