HLA-G monoclonal antibody, clone F21-21
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More Files
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Flow Cytometry
Chicken peripheral blood mononuclear cells were double-stained with HLA-G monoclonal antibody, clone F21-21 (FITC) and mouse anti-chicken CD3-R-PE. Small lymphocytes were gated using light scatter criteria and analyzed on a FACScan™ flow cytometer (BDB, San Jose, CA).
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Specification
Product Description
Mouse monoclonal antibody raised against native HLA-G.
Immunogen
Native purified from white blood cells from chicken plasma.
Host
Mouse
Reactivity
Chicken
Specificity
The beta2-microglobulin subunit (Mr ~ 14 KDa) of avian MHC Class I molecules.
Form
Liquid
Isotype
IgG1
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
Flow Cytometry
Chicken peripheral blood mononuclear cells were double-stained with HLA-G monoclonal antibody, clone F21-21 (FITC) and mouse anti-chicken CD3-R-PE. Small lymphocytes were gated using light scatter criteria and analyzed on a FACScan™ flow cytometer (BDB, San Jose, CA). -
Gene Info — HLA-G
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Publication Reference
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T cell precursor migration towards beta 2-microglobulin is involved in thymus colonization of chicken embryos.
Dunon D, Kaufman J, Salomonsen J, Skjoedt K, Vainio O, Thiery JP, Imhof BA.
The EMBO Journal 1990 Oct; 9(10):3315.
Application：Flow Cyt, Chicken, Chicken red blood cells.
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Isolation and characterization of chicken and turkey beta 2-microglobulin.
Skjodt K, Welinder KG, Crone M, Verland S, Salomonsen J, Simonsen M.
Molecular Immunology 1986 Dec; 23(12):1301.
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T cell precursor migration towards beta 2-microglobulin is involved in thymus colonization of chicken embryos.
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