CD4A monoclonal antibody, clone 74-12-4
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More Files
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Flow Cytometry
Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and double-stained with CD4A monoclonal antibody, clone 74-12-4 (PE) and mouse anti-pig CD8a-FITC. Lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Mouse monoclonal antibody raised against CD4A.
Immunogen
dd Miniature swine thymocytes.
Host
Mouse
Reactivity
Pig
Specificity
Porcine CD4a, Mr 55 KDa.
Form
Liquid
Isotype
IgG2b
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Immunoprecipitation
Flow Cytometry
Peripheral blood mononuclear cells were isolated from heparinized pig blood on Ficoll-Hypaque density gradients and double-stained with CD4A monoclonal antibody, clone 74-12-4 (PE) and mouse anti-pig CD8a-FITC. Lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Publication Reference
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Human anti-porcine xenogeneic T cell response. Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition.
Yamada K, Sachs DH, DerSimonian H.
Journal of Immunology 1995 Dec; 55(11):5249.
Application：Flow Cyt, Human, Porcine, Lymphoid cells, Macrophages.
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In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine.
Smith CV, Sablinski T, Arn JS, Myers DE, Rosengard BR, Uckun FM, Sachs DH.
Journal of Immunotherapy with Emphasis on Tumor Immunology 1994 Aug; 16(2):105.
Application：Flow Cyt, Pig, Lymphocytes, Peripheral blood lymphocytes.
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Characterization of a polymorphism of CD4 in miniature swine.
Sundt TM 3rd, LeGuern C, Germana S, Smith CV, Nakajima K, Lunney JK, Sachs DH.
The Journal of Immunology 1992 May; 148(10):3195.
Application：Flow Cyt, Mouse, Splenocytes.
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Human anti-porcine xenogeneic T cell response. Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition.
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