Hspa5 monoclonal antibody, clone 76-E6
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More Files
- More Functions
Western Blot
Recombinant hamster Hspa5 mutant (HCB1-, lane 1), full-length recombinant hamster Hspa5 (Lane 2) and total cell lysate from Ag8.653, a mouse myeloma cell line (Lane 3) were resolved by a 10% SDS-PAGE. The proteins were transferred onto a PVDF membrane and incubated with Hspa5 monoclonal antibody, clone 76-E6 (Biotin) followed by Streptavidin-HRP. The blot was then developed using ECL Western blot detection reagents (Amersham Life Science, Arlington Heights, IL). Note : We are grateful to Dr. Linda Hendershot for the recombinant hamster Hspa5 proteins and for helpful suggestions.
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Specification
Product Description
Rat monoclonal antibody raised against Hspa5.
Immunogen
Mouse BiP:u chain complexes.
Host
Rat
Reactivity
Mouse
Specificity
BiP (Immunoglobulin heavy chain binding protein, Mr 78 KDa).
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In 100 mM BBS, pH 8.2
Storage Instruction
Store at 4°C.
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Applications
Western Blot
Recombinant hamster Hspa5 mutant (HCB1-, lane 1), full-length recombinant hamster Hspa5 (Lane 2) and total cell lysate from Ag8.653, a mouse myeloma cell line (Lane 3) were resolved by a 10% SDS-PAGE. The proteins were transferred onto a PVDF membrane and incubated with Hspa5 monoclonal antibody, clone 76-E6 (Biotin) followed by Streptavidin-HRP. The blot was then developed using ECL Western blot detection reagents (Amersham Life Science, Arlington Heights, IL). Note : We are grateful to Dr. Linda Hendershot for the recombinant hamster Hspa5 proteins and for helpful suggestions.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Hspa5
Entrez GeneID
14828Gene Name
Hspa5
Gene Alias
78kDa, AL022860, AU019543, Bip, D2Wsu141e, D2Wsu17e, Grp78, Hsce70, SEZ-7, Sez7, mBiP
Gene Description
heat shock protein 5
Gene Ontology
HyperlinkGene Summary
78 kDa|heat shock 70kD protein 5 (glucose-regulated protein)|heat shock 70kD protein 5 (glucose-regulated protein
Other Designations
OTTMUSP00000012896|OTTMUSP00000012897|glucose regulated protein, 78 kDa|heat shock 70kD protein 5 (glucose-regulated protein)|heat shock 70kD protein 5 (glucose-regulated protein, 78kD)
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Publication Reference
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The role of molecular chaperones in protein folding.
Hendrick JP, Hartl FU.
FASEB Journal 1995 Dec; 9(15):1559.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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BiP (GRP78), an essential hsp70 resident protein in the endoplasmic reticulum.
Haas IG.
Experientia 1994 Nov; 50(11-12):1012.
Application：Func, IF, IP, WB-Ce, WB-Re, WB-Tr, Human, Mouse, Rat, Endoplasmic reticulum, Mammalian cells.
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Molecular chaperones in the processing and presentation of antigen to helper T cells.
Pierce SK.
Experientia 1994 Nov; 50(11-12):1026.
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The role of molecular chaperones in protein folding.
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