Mouse monoclonal antibody raised against partial recombinant CENPF.
Recombinant GST fusion protein corresponding to amino acids 1759-2093 of human CENPF.
This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression.
Western Blot (1-10 ug/mL) Immunoprecipitation (1-10 ug/mL) Immunohistochemistry (Frozen sections) (10 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL) The optimal working dilution should be determined by the end user.
In PBS, pH 7.4
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq
AH antigen,CENP-F kinetochore protein,OTTHUMP00000035028,cell-cycle-dependent 350K nuclear protein,centromere protein F,mitosin