CENPF monoclonal antibody, clone 14C10
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant CENPF.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 1759-2093 of human CENPF.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression.
Form
Liquid
Isotype
IgG2a, kappa
Recommend Usage
Western Blot (1-10 ug/mL)
Immunoprecipitation (1-10 ug/mL)
Immunohistochemistry (Frozen sections) (10 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunoprecipitation
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Gene Info — CENPF
Entrez GeneID
1063Gene Name
CENPF
Gene Alias
CENF, PRO1779, hcp-1
Gene Description
centromere protein F, 350/400ka (mitosin)
Omim ID
600236Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq
Other Designations
AH antigen|CENP-F kinetochore protein|OTTHUMP00000035028|cell-cycle-dependent 350K nuclear protein|centromere protein F|mitosin
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Interactome
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Disease
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Publication Reference
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Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression.
Zhu X, Mancini MA, Chang KH, Liu CY, Chen CF, Shan B, Jones D, Yang-Feng TL, Lee WH.
Molecular and Cellular Biology 1995 Sep; 15(9):5017.
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Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression.
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