BRCA1 monoclonal antibody, clone 3A2
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Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant BRCA1.
Immunogen
Recombinant GST fusion protein corresponding to amino acids 762-1315 of human BRCA1.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes full-length BRCA1, a 220 kD nuclear phosphoprotein, and does not recognize the Deltaexon 11 splice variant.
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
Western Blot (1-10 ug/mL)
Immunoprecipitation (1-10 ug/mL)
Immunohistochemistry (1-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
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Gene Info — BRCA1
Entrez GeneID
672Gene Name
BRCA1
Gene Alias
BRCAI, BRCC1, IRIS, PSCP, RNF53
Gene Description
breast cancer 1, early onset
Omim ID
113705Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq
Other Designations
BRCA1/BRCA2-containing complex, subunit 1|breast and ovarian cancer susceptibility protein 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor.
Chen CF, Li S, Chen Y, Chen PL, Sharp ZD, Lee WH.
The Journal of Biological Chemistry 1996 Dec; 271(51):32863.
Application:WB-Tr, Human, DU 145 cells.
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BRCA1 is a 220-kDa nuclear phosphoprotein that is expressed and phosphorylated in a cell cycle-dependent manner.
Chen Y, Farmer AA, Chen CF, Jones DC, Chen PL, Lee WH.
Cancer Research 1996 Jul; 56(14):3168.
Application:IF, IP, WB-Ce, WB-Tr, Human, Insect, HBL100, SF9, T24 cells.
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Aberrant subcellular localization of BRCA1 in breast cancer.
Chen Y, Chen CF, Riley DJ, Allred DC, Chen PL, Von Hoff D, Osborne CK, Lee WH.
Science 1995 Nov; 270(5237):789.
Application:IF, IHC, IP, WB-Ce, Human, HBL100 cells, Human breast cancer.
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The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor.
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