Cd3e monoclonal antibody, clone C363.29B (APC)
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More Files
- More Functions
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd3e monoclonal antibody, clone C363.29B (FITC) (Cat # MAB6100) and Ptprc monoclonal antibody, clone RA3-6B2 (PE) (Cat # MAB5722), gated on small lymphocytes and analyzed using a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against Cd3e.
Immunogen
H-2Kb specific mouse cytotoxic T lymphocyte clone BM10-37.
Host
Rat
Reactivity
Mouse
Specificity
Specificity Mouse CD3ε (Mr 25 kD)
Form
Liquid
Conjugation
APC
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c mesenteric lymph nodes were double-stained with Cd3e monoclonal antibody, clone C363.29B (FITC) (Cat # MAB6100) and Ptprc monoclonal antibody, clone RA3-6B2 (PE) (Cat # MAB5722), gated on small lymphocytes and analyzed using a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd3e
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Publication Reference
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Differential effect of interleukin 1 on naive and memory CD4+ T cells.
Luqman M, Greenbaum L, Lu D, Bottomly K.
European Journal of Immunology 1992 Jan; 22(1):95.
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Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
Payer E, Elbe A, Stingl G.
Journal of Immunology 1991 Apr; 146(8):2536.
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Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice.
Ernst DN, Hobbs MV, Torbett BE, Glasebrook AL, Rehse MA, Bottomly K, Hayakawa K, Hardy RR, Weigle WO.
Journal of Immunology 1990 Sep; 145(5):1295.
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Differential effect of interleukin 1 on naive and memory CD4+ T cells.
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