Itga4 monoclonal antibody, clone PS/2 (Biotin)
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Specification
Product Description
Rat monoclonal antibody raised against native Itga4.
Immunogen
Native purified from P815 mastocytoma cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity CD49d, the alpha4 chain (100 KDa) of the VLA-4 integrin heterodimer (Mr 150-KDa)
Form
Liquid
Conjugation
Biotin
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Flow Cytometry
Cells from BALB/c bone marrow were stained with Itga4 monoclonal antibody, clone PS/2 (FITC) (Cat # MAB6097), following which lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Itga4
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Publication Reference
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Alpha 4 integrin directs virus-activated CD8+ T cells to sites of infection.
Christensen JP, Andersson EC, Scheynius A, Marker O, Thomsen AR.
Journal of Immunology 1995 May; 154(10):5293.
Application:Flow Cyt, Mouse , Mouse spleen cells.
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Expression of murine VCAM-1 in vitro and in different models of inflammation in vivo: correlation with immigration of monocytes.
Henseleit U, Steinbrink K, Sunderkotter C, Goebeler M, Roth J, Sorg C.
Experimental Dermatology 1994 Dec; 3(6):249.
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Decreased adhesion to endothelial cells and matrix proteins of H-2Kb gene transfected tumour cells.
Lauri D, De Giovanni C, Biondelli T, Lalli E, Landuzzi L, Facchini A, Nicoletti G, Nanni P, Dejana E, Lollini PL.
British Journal of Cancer 1993 Nov; 68(5):862.
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Alpha 4 integrin directs virus-activated CD8+ T cells to sites of infection.
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