Vcam1 monoclonal antibody, clone M/K-2 (Biotin)
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More Files
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Flow Cytometry
BALB/c bone marrow cells were stained with Vcam1 monoclonal antibody, clone M/K-2 (FITC) (Cat # MAB6094), following which large cells were gated and analyzed by flow cytometry.
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Specification
Product Description
Rat monoclonal antibody raised against Vcam1.
Immunogen
Balb/3T3, +/+ 2.4 cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity CD106/VCAM-1 (~Mr 100-KDa)
Form
Liquid
Conjugation
Biotin
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
BALB/c bone marrow cells were stained with Vcam1 monoclonal antibody, clone M/K-2 (FITC) (Cat # MAB6094), following which large cells were gated and analyzed by flow cytometry. -
Gene Info — Vcam1
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Publication Reference
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Inhibition of T cell costimulation by VCAM-1 prevents murine graft-versus-host disease across minor histocompatibility barriers.
Schlegel PG, Vaysburd M, Chen Y, Butcher EC, Chao NJ.
Journal of Immunology 1995 Oct; 155(8):3856.
Application：Func, Mouse, Bone marrow cells, Lymphocytes, Spleen cells.
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A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture.
Miyake K, Medina K, Ishihara K, Kimoto M, Auerbach R, Kincade PW.
The Journal of Cell Biology 1991 Aug; 114(3):557.
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Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis.
Miyake K, Weissman IL, Greenberger JS, Kincade PW.
The Journal of Experimental Medicine 1991 Mar; 173(3):599.
Application：Flow Cyt, Mouse, 7OZ/3, B815, BM-2, WEHI231 cells.
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Inhibition of T cell costimulation by VCAM-1 prevents murine graft-versus-host disease across minor histocompatibility barriers.
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