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Ptprc monoclonal antibody, clone I3/2.3 (SPRD)
Ptprc monoclonal antibody, clone I3/2.3 (SPRD)
Catalog # :
MAB5941
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Liberia
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Paraguay
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Specification
Gene Info
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Specification
Product Description:
Rat monoclonal antibody raised against native Ptprc.
Immunogen:
Native purified Ptprc from T1M1 (Thy-1-C) cells.
Host:
Rat
Reactivity:
Mouse
Specificity:
Specificity Mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms)
Form:
Liquid
Conjugation:
SPRD
Isotype:
IgG2b
Recommend Usage:
Flow Cytometry (0.2 ug/10
6
cells)
The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Datasheet:
Download
Publication Reference
1.
CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development.
Trowbridge IS, Thomas ML.Annu Rev Immunol. 1994;12:85-116.
2.
Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies.
Johnson P, Greenbaum L, Bottomly K, Trowbridge IS.J Exp Med. 1989 Mar 1;169(3):1179-84.
3.
Interspecies spleen-myeloma hybrid producing monoclonal antibodies against mouse lymphocyte surface glycoprotein, T200.
Trowbridge IS.J Exp Med. 1978 Jul 1;148(1):313-23.
Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were double-stained with Ptprc monoclonal antibody, clone I3/2.3 (FITC) (Cat # MAB5936) and rat anti-mouse CD4-FITC. Small lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
Application Image
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
enlarge
Gene Information
Entrez GeneID:
19264
Gene Name:
Ptprc
Gene Alias:
B220,CD45R,Cd45,Ly-5,Lyt-4,T200,loc
Gene Description:
protein tyrosine phosphatase, receptor type, C
Gene Ontology:
Hyperlink
Other Designations:
lymphocyte common antigen
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