Cd40 monoclonal antibody, clone 1C10 (PE)
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More Files
- More Functions
Flow Cytometry
Cells from BALB/c spleen were stained with Cd40 monoclonal antibody, clone 1C10 (FITC) (Cat # MAB5931) and Cd3e monoclonal antibody, clone 145-2C11 (PE) (Cat # MAB5909), gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against partial Cd40.
Immunogen
Extracelluar domain of mouse Cd40.
Host
Rat
Reactivity
Mouse
Specificity
Specificity Mouse CD40, Mr 45-50 KDa
Form
Liquid
Conjugation
PE
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (0.5 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Cells from BALB/c spleen were stained with Cd40 monoclonal antibody, clone 1C10 (FITC) (Cat # MAB5931) and Cd3e monoclonal antibody, clone 145-2C11 (PE) (Cat # MAB5909), gated on small lymphocytes and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd40
Entrez GeneID
21939Gene Name
Cd40
Gene Alias
AI326936, Bp50, GP39, HIGM1, IGM, IMD3, T-BAM, TRAP, Tnfrsf5, p50
Gene Description
CD40 antigen
Gene Ontology
HyperlinkGene Summary
member 5
Other Designations
OTTMUSP00000017667|OTTMUSP00000017739|T-cell differentiation antigen|tumor necrosis factor receptor superfamily, member 5
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Publication Reference
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Monoclonal antibodies to murine CD40 define two distinct functional epitopes.
Heath AW, Wu WW, Howard MC.
European Journal of Immunology 1994 Aug; 24(8):1828.
Application：Flow Cyt, Mouse, Mouse B cells, L cells.
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Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies.
Hasbold J, Johnson-Leger C, Atkins CJ, Clark EA, Klaus GG.
European Journal of Immunology 1994 Aug; 24(8):1835.
Application：Flow Cyt, Mouse, BHK cells.
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Antibodies to murine CD40 protect normal and malignant B cells from induced growth arrest.
Santos-Argumedo L, Gordon J, Heath AW, Howard M.
Cell 1994 Jul; 156(2):272.
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Monoclonal antibodies to murine CD40 define two distinct functional epitopes.
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