Cd38 monoclonal antibody, clone 90 (Biotin)
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More Files
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Flow Cytometry
Cells from BALB/c spleen were double-stained with Cd38 monoclonal antibody, clone 90 (FITC) (Cat # MAB5926) and Cd3e monoclonal antibody, clone 145-2C11 (PE) (Cat # MAB5909). Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Rat monoclonal antibody raised against native Cd38.
Immunogen
Native purified Cd38 from mouse bone marrow pre-B cells.
Host
Rat
Reactivity
Mouse
Specificity
Specificity CD38 (Mr 42-KDa), an immunoregulatory ectoenzyme
Form
Liquid
Conjugation
Biotin
Isotype
IgG2a, kappa
Recommend Usage
Flow Cytometry (1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Cells from BALB/c spleen were double-stained with Cd38 monoclonal antibody, clone 90 (FITC) (Cat # MAB5926) and Cd3e monoclonal antibody, clone 145-2C11 (PE) (Cat # MAB5909). Small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd38
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Publication Reference
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Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38.
Howard M, Grimaldi JC, Bazan JF, Lund FE, Santos-Argumedo L, Parkhouse RM, Walseth TF, Lee HC.
Science 1993 Nov; 262(5136):1056.
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Expression cloning of a cDNA encoding a novel murine B cell activation marker. Homology to human CD38.
Harada N, Santos-Argumedo L, Chang R, Grimaldi JC, Lund FE, Brannan CI, Copeland NG, Jenkins NA, Heath AW, Parkhouse RM, et al..
Journal of Immunology 1993 Sep; 151(6):3111.
Application：Flow Cyt, Monkey, COS-7 cells.
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A B lymphocyte surface molecule mediating activation and protection from apoptosis via calcium channels.
Santos-Argumedo L, Teixeira C, Preece G, Kirkham PA, Parkhouse RM.
Journal of Immunology 1993 Sep; 151(6):3119.
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Formation and hydrolysis of cyclic ADP-ribose catalyzed by lymphocyte antigen CD38.
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