Cd3e monoclonal antibody, clone 145-2C11 (PE)
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More Files
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Flow Cytometry
BALB/c spleen cells were double-stained with Cd3e monoclonal antibody, clone 145-2C11 (FITC) (Cat # MAB5907) and rat anti-mouse CD19-R-PE. Lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
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Specification
Product Description
Hamster monoclonal antibody raised against Cd3e.
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37.
Host
Hamster
Reactivity
Mouse
Specificity
Mouse CD3ε (Mr 25 KDa).
Form
Liquid
Conjugation
PE
Isotype
IgG, kappa
Recommend Usage
Flow Cytometry (0.1 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunoprecipitation
Flow Cytometry
BALB/c spleen cells were double-stained with Cd3e monoclonal antibody, clone 145-2C11 (FITC) (Cat # MAB5907) and rat anti-mouse CD19-R-PE. Lymphocytes were gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA). -
Gene Info — Cd3e
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Publication Reference
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Differential activation of antigen-stimulated suicide and cytokine production pathways in CD4+ T cells is regulated by the antigen-presenting cell.
Wang R, Murphy KM, Loh DY, Weaver C, Russell JH.
Journal of Immunology 1993 May; 150(9):3832.
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Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death.
Ucker DS, Meyers J, Obermiller PS.
Journal of Immunology 1992 Sep; 149(5):1583.
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Circulating CD3+/T cell receptor V gamma 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
Payer E, Elbe A, Stingl G.
Journal of Immunology 1991 Apr; 146(8):2536.
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Differential activation of antigen-stimulated suicide and cytokine production pathways in CD4+ T cells is regulated by the antigen-presenting cell.
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