MAPK1/MAPK3 monoclonal antibody, clone SB46b (HRP)
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Western Blot
Human PBMC (Lane 1) and spleen (Lane 2) lysate was stained with MAPK1/3 monoclonal antibody, clone SB46b. Goat anti-Mouse IgG2a was used to develop.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant MAPK1/MAPK3.
Immunogen
Recombinant protein corresponding to human MAPK1/MAPK3.
Host
Mouse
Reactivity
Human
Specificity
Reacts with human Erk 1 and human Erk2.
Form
Liquid
Conjugation
HRP
Concentration
Lot specific
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (50% glycerol)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Human PBMC (Lane 1) and spleen (Lane 2) lysate was stained with MAPK1/3 monoclonal antibody, clone SB46b. Goat anti-Mouse IgG2a was used to develop.Immunoprecipitation
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Gene Info — MAPK1
Entrez GeneID
5594Gene Name
MAPK1
Gene Alias
ERK, ERK2, ERT1, MAPK2, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk
Gene Description
mitogen-activated protein kinase 1
Omim ID
176948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174492|extracellular signal-regulated kinase 2|extracellular signal-regulated kinase-2|mitogen-activated protein kinase 2|protein tyrosine kinase ERK2
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Gene Info — MAPK3
Entrez GeneID
5595Gene Name
MAPK3
Gene Alias
ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3
Gene Description
mitogen-activated protein kinase 3
Omim ID
601795Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000174538|OTTHUMP00000174540|extracellular signal-regulated kinase 1|extracellular signal-related kinase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking.
Xu Be, Stippec S, Robinson FL, Cobb MH.
The Journal of Biological Chemistry 2001 May; 276(28):26509.
Application：Immunoblotting, Human, HEK 293 cells.
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Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions.
Pearson G, Robinson F, Beers Gibson T, Xu BE, Karandikar M, Berman K, Cobb MH.
Endocrine Reviews 2001 Apr; 22(2):153.
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Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.
Charest DL, Mordret G, Harder KW, Jirik F, Pelech SL.
Molecular and Cellular Biology 1993 Aug; 13(8):4679.
Application：WB, Bacteria, Bacteria lysates.
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Hydrophobic as well as charged residues in both MEK1 and ERK2 are important for their proper docking.
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