VSIG1 monoclonal antibody, clone CT1 (PE)
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More Files
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Flow Cytometry
Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and VSIG1 monoclonal antibody, clone CT1 (FITC) (Cat # MAB5856). Analysis was performed on a FACScan™ flow cytometer (BDB, San Jose, CA) using a light scatter gate to identify small lymphocytes.
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Specification
Product Description
Mouse monoclonal antibody raised against native VSIG1.
Immunogen
Native purified from chicken thymocytes.
Host
Mouse
Reactivity
Chicken
Specificity
ChT1 antigen (Mr 63/104 KDa) on chicken thymocytes.
Form
Liquid
Conjugation
PE
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
Chicken thymocytes were double stained with mouse anti-chicken CD3-PE and VSIG1 monoclonal antibody, clone CT1 (FITC) (Cat # MAB5856). Analysis was performed on a FACScan™ flow cytometer (BDB, San Jose, CA) using a light scatter gate to identify small lymphocytes. -
Gene Info — VSIG1
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Publication Reference
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ChT1, an Ig superfamily molecule required for T cell differentiation.
Katevuo K, Imhof BA, Boyd R, Chidgey A, Bean A, Dunon D, Gobel TW, Vainio O.
Journal of Immunology 1999 May; 162(10):5685.
Application：Flow Cyt, Chicken, Cells from chickens’ bone marrow, spleen, thymus, PBL .
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Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation.
Kong F, Chen CH, Cooper MD.
Immunity 1998 Jan; 8(1):97.
Application：Flow Cyt, Chicken, Thymocytes.
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Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker.
Houssaint E, Diez E, Jotereau FV.
European Journal of Immunology 1985 Mar; 15(3):305.
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ChT1, an Ig superfamily molecule required for T cell differentiation.
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