MCAM monoclonal antibody, clone C264 (PE)
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More Files
- More Functions
Flow Cytometry
Thymocytes from a young chicken were isolated and incubated with MCAM monoclonal antibody, clone C264. The cells were then stained with goat anti-mouse IgG2b-FITC and analyzed on a FACScan™ flow cytometer (BDB, San Jose, CA).
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Specification
Product Description
Mouse monoclonal antibody raised against native MCAM.
Immunogen
Native purified chicken MCAM.
Host
Mouse
Reactivity
Chicken
Specificity
Chicken MCAM, Mr 98 KDa (protein core =84 KDa).
Form
Liquid
Conjugation
PE
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (0.2 ug/106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
Thymocytes from a young chicken were isolated and incubated with MCAM monoclonal antibody, clone C264. The cells were then stained with goat anti-mouse IgG2b-FITC and analyzed on a FACScan™ flow cytometer (BDB, San Jose, CA). -
Gene Info — MCAM
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Publication Reference
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HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors.
Vainio O, Dunon D, Aissi F, Dangy JP, McNagny KM, Imhof BA.
The Journal of Cell Biology 1996 Dec; 135(6 Pt 1):1655.
Application：Flow Cyt, IHC-Fr, Chicken, E13.5 H.B19 bone marrow, embryonic tissues, spleen, thymus cells.
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HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors.
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